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Exptl. Biol. Med. Sei. 19, 185 (1941). VI. ESTIMATION 25 Lampen and Peterson48 have described a rapid turbidimetric method involving a basal medium containing Norit-treated casein as the only semipurified component. Anaerobic conditions for the growth of Clostridium acetobutylicum were established by the use of sodium hydrosulfite (Na 2 S 2 0 4 ) and reduced iron as components of the basal medium, and by incubating the assays in an "oat jar," where respiration of the oats establishes a partial pressure of C0 2 .

Unna and W. L. Sampson, Proc. Soc. Exptl. Biol. Med. 45, 309 (1940). 68 G. A. Emerson and H. M. Evans, Proc. Soc. Exptl. Biol. Med. 46, 655 (1941). 69 K. Unna, Am. J. Physiol. 133, 473 (1941). 70 C. A. Elvehjem, L. M. Henderson, S. Black, and E. Nielsen, J Biol. Chem. 140, xxxvi (1941). 71 R. R. Williams, Science 92, 561 (1940). 72 D. V. Frost, R. C. Moore, and F. P. Dann, Proc. Soc. Exptl. Biol. Med. 46, 507 (1941). 73 G. J. Martin, Am. J. Physiol. 136, 124 (1942). 74 K. Unna, G. V. Richards, and W.

147,485 (1943). 44 R. C. Thompson, E. R. Isbell, and H. K. Mitchell, / . Biol. Chem. 148, 281 (1943). 45 S. C. Agarwala and W. H. Peterson, Arch. Biochem. 27, 304 (1950). 46 G. W. Beadle, Physiol. Revs. 25, 643 (1945). 47 N. Fries, Nature 155, 757 (1945). 48 C. B. Anfinsen, Q. M. Geiman, R. W. McKee, R. A. Ormsbee, and E. G. Ball, / . Exptl. Med. 84, 607 (1946). 49 R. C. Thompson, J. Bacteriol. 46, 99 (1943). 50 V. G. Lilly and L. H. Leonian, / . Bacteriol. 50, 383 (1945). 81 S. H. Hutner, J. Bacteriol.

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