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By B C Currell, R C E Dam-Mieras

This booklet discusses the subsequent: uptake of nutrition, nitrogen and sulphur assimilation, amino acid and nucleotide biosynthesis, the biosynthesis of carbohydrates, the mixing and legislation of metabolism and keep watch over of metabolic pathway flux

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Extra info for Biosynthesis and the Integration of Cell Metabolism

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The energised carrier has a reduced affinity for substrate on the cytoplasmic side of the membrane. Π Would you expect a reduced affinity for substrate on the cytoplasmic side of the membrane to decrease the rate of transport? Explain your answer. The correct answer is no. This is because a reduced affinity on this side of the membrane would enhance the breakdown of the carrier-substrate complex. 7). It was originally thought that carriers might diffuse across the membrane, but studies have revealed that membrane proteins diffuse across the membrane only very slowly or not at all.

A common feature is to omit positional information (eg 3', 50. 2 Decide whether each of the following statements is true or false and justify your decisions: 1) Organisms able to utilise sulphate are also likely to be able to utilise sulphite and hydrogen sulphide. 2) NADPH is required for the reaction of hydrogen sulphide to form cysteine. 3) Serine can serve as a source of carbon, nitrogen and sulphur for cells. 4) Assimilation of sulphate is more energetically demanding on cells than the assimilation of cysteine.

Activated sulphate The pathway of assimilatory sulphate reduction to H2S has four stages. · Synthesis of activated sulphate. The sulphate is first converted to adenosine phosphosulphate. This involves the transfer of adenosine monophosphate from ATP to sulphate and is catalysed by the enzyme sulphate adenylyl-transferase (also called ATP sulphurylase). ATP + SOl" <^ adenosine 5'-phosphosulphate + 2PPL The enzyme adenylylsulphate kinase then further activates the sulphate by transfer of a phosphate from ATP to form phosphoadenosine phosphosulphate (adenosine 3'-phosphate 5'-phosphosulphate).

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