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64, 37-43. Valdivia, R. H. & Falkow, S. 1996 Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid inducible promoters by differential fluorescence induction. Mol. Microbiol. 23, 367-378. Valdivia, R. H. & Falkow, S. 1997a Fluorescence-based isolation of bacterial genes expressed within host cells. Science 277, 2007-2011. Valdivia, R. H. & Falkow, S. 19976 Probing bacterial gene expression within host cells. Trends Microbiol. 5, 360-363. , Ferguson, D. J. , Acktman, M.

E. 1996 Salmonella spp. are cytotoxic for cultured macrophages. Mol. Microbiol. 21, 1101-1115. Chiang, S. L. & Mekalanos, J. J. 1998 Use of signature-tagged transposon mutagenesis to identify Vibrio cholerae genes critical for colonization. Mol. Microbiol. 27, 797-805. Clarke, M. , Reece, K. , Hirst, B. H. & Jepson, M. A. 1996 Invasion of murine intestinal M cells by Salmonella typhimurium inv mutants severely deficient for invasion of cultured cells. Infect. Immun. 64, 4363-4368. Coates, A. R.

Gleeson, C. & Holden, D. W. 1996 Identification of a virulence locus encoding a second type III secretion system in Salmonella typhimurium. Proc. Natl Acad. Sci. USA 93, 2593-2597. Skorupski, K. & Taylor, R. K. 1997 Control of the ToxR virulence regulon in Vibrio cholerae by environmental stimuli. Mol. Microbiol. 25, 1003-1009. Smith, H. 1990 Pathogenicity and the microbe in vivo. J. Gen. Microbiol. 136, 377-393. Smith, H. 1995 The revival of interest in mechanisms of bacterial pathogenicity.

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