By Rodolfo Paoletti, Dr. David Kritchevsky
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Extra info for Advances in lipid research. Volume 3
Low-Density Lipoprotein Isolation B. High-Density Lipoprotein Isolation C. Analytical Ultracentrifugal Analysis of Lipoproteins III. A Generalized Method for the Computer Analysis of Lip oprotein Distributions IV. Application of Computer Analysis to a Series of Adult Normal Males and Females A. Lipoprotein Profiles for an Adult Male and Female Population B. Preliminary Relationships Observed between Sf Rate and Lipoprotein Concentrations C. Preliminary Relationships Observed between Lipo protein Concentration, Lipoprotein Composition, and Other Parameters V.
Although the theory and mathematical treatment of the 1 This work was supported by Research Grants HE 01882-09 and HE 02029-09 from the National Heart Institute, Public Health Service, Bethesda, Maryland, and by the United States Atomic Energy Commission. 25 26 E W I N G E T AL. behavior of homogeneous molecular systems in the ultracentrifuge have been elegantly presented (3), the analysis of paucidisperse and polydisperse systems continues to present considerable difficulty (4, 5). This problem is particularly relevant if one is attempting to analyze ultracentrifugally a distribution of macromolecules that may differ both in particle size and in hydrated density.
This permits uniform treatment of the data regardless of the num ber and location of the discontinuities of x. However, as this is a purely internal program operation, no attempt is made to include these features in our notation. Using the ct as calculated above, the program can easily generate the cumulative concentration at each fa by: i The preliminary integral curve consists of these ct paired with fa or x*. Its accuracy is, of course, limited by the number of intervals selected. How ever, with 29 intervals evaluated from three frames over the flotation range Sf = 0 to Sf = 400, our calculated concentrations have been within approximately 3% of the values obtained by planimetry (see Table I).